Retention volume
This is the total volume of solvent needed to cause component elution. This is where Vr is the volume of the solvent moved through the column and Fv and tr are the flow rate and retention time. |
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Partition coefficient
The partition coefficient is a measure of how separated the components are separated in solution. |
Retention factor
The retention factor is a measure of the retention of the component compared to the void time. The retention factor and partition coefficient can be directly related. |
Separation factor
The separation factor is a measure how well two components have separated on the column.
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Van Deemter equation
The Van Deemter equation gives the theoretical plate height for a system and is related to the flow rate in many cases.
The Van Deemter equation gives the theoretical plate height for a system and is related to the flow rate in many cases.
Eddy diffusion
This is not related to the flow rate. It is purely based on how the sample flows through the stationary phase. It is also affected by the eddy currents formed around the edges of the column according to the Laminar flow model. |
Molecular (longitudinal) diffusion
This is the usual diffusion model. Based on the random walk of molecules away from a high density to a low density. |
Mass transfer
A faster partitioning process results in decreased band broadening. With increased adsorption/desorption time there is an increase in the amount |
Where u is the flow rate.